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Registros recuperados: 81 | |
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Campos,Tatiana de; Cunha,Marcia Oliveira da; Sousa,Adna Cristina Barbosa de; Teixeira,Renata Beltrão; Raposo,Andrea; Sebbenn,Alexandre Magno; Wadt,Lucia Helena de Oliveira. |
The objective of this work was to estimate the mating system parameters of a andiroba (Carapa guianensis) population using microsatellite markers and the mixed and correlated mating models. Twelve open‑pollinated progeny arrays of 15 individuals were sampled in an area with C. guianensis estimated density of 25.7 trees per hectare. Overall, the species has a mixed reproductive system, with a predominance of outcrossing. The multilocus outcrossing rate (t m = 0.862) was significantly lower than the unity, indicating that self‑pollination occurred. The rate of biparental inbreeding was substantial (t m ‑ t s = 0.134) and significantly different from zero. The correlation of selfing within progenies was high (r s = 0.635), indicating variation in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carapa guianensis; Allogamy; Conservation; Genetic breeding; Microsatellite; Population size. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000500006 |
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Barbosa,Ana Angélica Leal; Sousa,Sandra Mara Bispo; Abé-Sandes,Kiyoko; Alonso,Carlos Alberto; Schneider,Vicente; Costa,Denise C.C.; Cavalli,Iglenir João; Azevêdo,Eliane Elisa Souza. |
The African descent population of the Bananal community in the Brazilian state of Bahia (BA) was characterized as a genetic isolate and analyzed for some short tandem repeat (STR) microsatellite autosomic polymorphic loci (CSF1PO, TH01, TPOX, F13A1, FESFPS and vWA). These genetic variants were further compared to data obtained from an urban sample from the town of Jequié (BA) regarding demographic and anthropogenetic aspects. The Bananal sample comprised 32 unrelated individuals whereas Jequié was represented by 76 individuals. The Bananal Negroid Phenotypic Index (NPI) was 0.98 and the Negroid Cultural Index (NCI) 0.24. Consanguineous marriages occurred at a frequency of 34.61% and the F value was 0.0126. All six loci studied were in Hardy-Weinberg... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite; African-descendant isolate. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100004 |
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Lallias, Delphine; Stockdale, Ruth; Lapegue, Sylvie; Boudry, Pierre; Beaumont, Andy. |
The European flat oyster Ostrea edulis and the blue mussel Mytilus edulis are both very valuable commercial species across Europe. Despite their economical importance, only a few microsatellite markers have so far been developed in those species. The aim of this study was to develop new microsatellites for O. edulis and M. edulis that could be used for population genetics studies, genetic variability assessment of stocks, parentage analysis or genetic and QTL mapping studies. For Ostrea edulis, an enriched library was made by ecogenics GmbH (Zurich, Switzerland) from size selected genomic DNA ligated into SAULA/SAULB-linker and enriched by magnetic bead selection with biotin-labelled (GT)13 and (CT)13 oligonucleotide repeats (Gautschi et al. 2000a,b). Of... |
Tipo: Text |
Palavras-chave: M13 tail; Blue mussel; European flat oyster; Microsatellite. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4536.pdf |
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Caballero,Susana; Steel,Debbie; Pallin,Logan; Botero-Acosta,Natalia; Felix,Fernando; Olavarría,Carlos; Diaz granados,Maria Claudia; Bessudo,Sandra; Friedlander,Ari; Scott Baker,C.. |
ABSTRACT We analysed 114 DNA samples collected in different locations within the stock G breeding grounds, including Ecuador and the northern and southern Pacific of Colombia. We genotyped 15 microsatellite loci, sexed all samples, and performed genotype comparisons. Genotype comparisons were done using a DNA register of previously genotyped individuals from the Colombian breeding ground and the Antarctic Peninsula feeding Area. We confirmed connectivity between feeding grounds in Antarctic Peninsula and breeding grounds in Colombia and Ecuador using microsatellite loci. We found recaptures within each sampling location in the same year, but we also found a few recaptures in the same locations between years both in breeding and feeding grounds.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Megaptera novaeangliae; Southeastern Pacific; Genotyping; Microsatellite; Migratory connectivity.. |
Ano: 2021 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612021000300031 |
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Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Kotoulas, G.; Bonhomme, Francois. |
Oysters, like many other marine species, are characterized by very high fertilities. Natural or hatchery populations, often present very low Ne/N ratios (Hedgecock, 1994). It can be explained by very high variances of their breeding success (Li and Hedgecock, 1998), the effective population size being estimated through the temporal variance of the allelic frequencies in natural populations (Hedgecock et al., 1992) or, more directly, through the estimated number of founders in hatchery populations (Launey, 1998). This document is a presentation of the results of a number of experiments, realised in the scope of the European program "GENEPHYS" (FAIR 95-421), and using microsatellite markers to analyse the parental contributions in the flat oyster Crassostrea... |
Tipo: Text |
Palavras-chave: Microsatellite; Succès reproducteur; Contributions parentales; Génétique; Crassostrea gigas; Huîtres. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/acte-3454.pdf |
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Pereira,Guilherme da Silva; Cazé,Ana Luíza Ramos; Silva,Michelle Garcia da; Almeida,Vanessa Cavalcante; Magalhães,Fernanda Oliveira da Cunha; Silva Filho,João Luís da; Barroso,Paulo Augusto Vianna; Hoffmann,Lúcia Vieira. |
Abstract: The objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gossypium hirsutum; Cultivar discrimination; Intraspecific polymorphism; Microsatellite. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000700571 |
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Santos,Camilla Alves; Farias,Flavio; Teixeira,Ana Karina; Rocha,João Luís; Guerrelhas,Ana Carolina; Freitas,Patrícia Domingues de. |
Abstract: The objective of this work was to assess polymorphisms within Litopenaeus vannamei candidate genes related to growth performance, and a possible association between weight genotypes and phenotypes in two shrimp closed lines. Three EST-SSR (serine-arginine, 60S ribosomal, and troponin) and one EST-SNP (crustacyanin) were selected and evaluated in L. vannamei and seven other marine shrimp species, besides two freshwater species. Genotypes had no effect on total body weight. Although no association was observed in the sampled population, these markers showed amplification for most of the evaluated species and, therefore, may be useful in further genetic studies on shrimp species. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidate genes; Crustacyanin; Microsatellite; SNP; Weight-association; 60S ribosomal. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100121 |
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De Noia, Michele; Telesca, Luca; Vendrami, David L. J.; Gokalp, Hatice K.; Charrier, Gregory; Harper, Elizabeth M.; Hoffman, Joseph I.. |
The soft-shell clam Mya arenaria is one of the most ancient invaders of European coasts and is present in many coastal ecosystems, yet little is known about its genetic structure in Europe. We collected 266 samples spanning a latitudinal cline from the Mediterranean to the North Sea and genotyped them at 12 microsatellite loci. In parallel, geometric morphometric analysis of shell outlines was used to test for associations between shell shape, latitude and genotype, and for a selection of shells we measured the thickness and organic content of the granular prismatic (PR), the crossed-lamellar (CL) and the complex crossed-lamellar (CCL) layers. Strong population structure was detected, with Bayesian cluster analysis identifying four groups located in the... |
Tipo: Text |
Palavras-chave: Mya arenaria; Soft-shell clam; Microsatellite; Population genetic structure; Phenotypic plasticity; Shell morphometrics. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00614/72600/71593.pdf |
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Machado,Mariana B.B.; Alencar,Maurício M.; Pereira,Andréa P.; Oliveira,Henrique N.; Casas,Eduardo; Coutinho,Luis L.; Regitano,Luciana C.A.. |
The objective of this work was to identify QTLs for liveweight in a candidate region of bovine chromosome 5. Half-sib families from two lines, one traditional and the other new, of Canchim beef cattle (5/8 Charolais + 3/8 Zebu) were genotyped for four microsatellite markers, including the microsatellite in the IGF-1 (insulin-like growth factor-1) promoter region. Significant differences in allele distribution between the two lines were found for three markers. Interval mapping analyses in this region indicated the presence of a QTL controlling birth weight (p < 0.05) and of a QTL influencing breeding value for yearling weight (p < 0.01) in the newer line of the breed. The previously identified interaction between the IGF-1 genotype and genetic group... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: QTL; Growth traits; Microsatellite; Bovine. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300008 |
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Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie. |
es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)... |
Tipo: Text |
Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf |
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Muths, Delphine; Tessier, Emmanuel; Gouws, G.; Craig, M.; Mwale, M.; Mwaluma, J.; Mwandya, A.; Bourjea, Jerome. |
The reef fish Myripristis berndti (Jordan & Everman 1903) is a pantropical species. A genetic analysis was conducted on 353 individuals from 10 localities distributed across the SW Indian Ocean (SWIO) in order to determine patterns of connectivity in the SWIO. Both the mtDNA sequences (711-bp cytochrome b sequences) and the microsatellites (8 newly developed loci) reveal spatial patterns of differentiation within the SWIO. There is, however, a discrepancy between the structure observed with each kind of marker. MtDNA revealed that 3 peripheral populations (NW Kenya, SE Reunion, and SW Europa) were isolated from the 7 more central populations, which form a more densely connected population network, while microsatellite data indicated a more restricted... |
Tipo: Text |
Palavras-chave: MtDNA; Microsatellite; Marine fish; SW Indian Ocean; Connectivity. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00073/18444/15974.pdf |
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Cowart, Dominique; Huang, Chunya; Arnaud-haond, Sophie; Carney, Susan L.; Fisher, Charles R.; Schaeffer, Stephen W.. |
The history of colonization and dispersal in fauna distributed among deep-sea chemosynthetic ecosystems remains enigmatic and poorly understood because of an inability to mark and track individuals. A combination of molecular, morphological and environmental data improves understanding of spatial and temporal scales at which panmixia, disruption of gene flow or even speciation may occur. Vestimentiferan tubeworms of the genus Escarpia are important components of deep -sea cold seep ecosystems, as they provide long-term habitat for many other taxa. Three species of Escarpia, Escarpia spicata [Gulf of California (GoC)], Escarpia laminata [Gulf of Mexico (GoM)] and Escarpia southwardae (West African Cold Seeps), have been described based on morphology, but... |
Tipo: Text |
Palavras-chave: Deep sea; Hydrocarbon seep; Microsatellite; Population structure; Siboglinid; Vestimentiferan tubeworm. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26354/24515.pdf |
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Diaz Almela, Elena. |
La structuration géographique des populations naturelles d'huître plate (Ostrea edulis) a été étudiée à partir de l'analyse du polymorphisme de conformation simple brin (SSCP), d'un fragment d'environ 300 pb du gène de l'ARNr mitochondrial 12S. les échantillons (13 localités, 507 individus) utilisés avaient fait l'objet d'une étude antérieure de structuration des populations avec 5 locus microsatellites. Il ont été analysés ici dans le but d'une comparaison des structurations observées sur marqueurs mitochondrial et nucléaire. La diversité moyenne intrapopulation du gène de l'ARNr 12S (Hn.b = 0,494) est presque la moitié de celle estimée avec les microsatellites (Hn.b = 0,904) bien que la variance entre populations soit beaucoup plus forte pour l'ADN... |
Tipo: Text |
Palavras-chave: Génétique population; Huître plate; Ostrea edulis; Microsatellite; ARNr mitochondrial 12S; Diversité génétique. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00033/14381/11671.pdf |
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Oliveira,Joelliton Domingos de; Igarashi,Maria Luiza Silveira de Paiva; Machado,Théa Mírian Medeiros; Miretti,Marcos Mateo; Ferro,Jesus Aparecido; Contel,Eucleia Primo Betioli. |
The genetic relationships and structure of fourteen goat (Capra hircus) populations were estimated based on genotyping data from 14 goat populations (n = 410 goats) at 13 microsatellite loci. We used analysis of molecular variance (AMOVA), principal component analysis (PCA) and F statistics (F IS, F IT and F ST) to evaluate the genetic diversity (Ho, He and ad) of the goats. Genetic distances between the 14 goat populations were calculated from allelic frequency data for the 13 microsatellite markers. Moderate differentiation was observed for the populations of the undefined breeds (including the Anglo-Nubian-M breed), the naturalized Brazilian breeds (Moxotó, Canindé), the exotic purebred breeds (Alpine, Saanen, Toggenbourg and Anglo-Nubian) and the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Microsatellite; Genetic distance; PCA; AMOVA; Genetic relationships; Genetic diversity; F statistics. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300010 |
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Melo,Bruno F.; Sato,Yoshimi; Foresti,Fausto; Oliveira,Claudio. |
The rio São Francisco basin contains many endemic species, such as Prochilodus argenteus and P. costatus, which have great commercial importance. However, information about the main recruitment sites and genetic studies containing extensive sampling of these species are scarce. To investigate the roles of the marginal lagoons in the maintenance of genetic variability and in the population structure, we analyzed six microsatellite loci in nine sampling groups of P. argenteusand five sampling groups of P. costatus. Our results showed high levels of genetic variability and low values of genetic differentiation for P. argenteus (FST =0.008, P< 0.05) and for P. costatus(FST =0.031, P < 0.05). In addition, high values of gene flow combined with a small... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation genetics; Fish migration; Microsatellite; Nursery area; Rio São Francisco basin. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300625 |
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Hervouet, Veronique. |
Les marqueurs génétiques très polymorphes sont des outils particulièrement intéressants pour l'analyse de contributions parentales. Dans notre étude, nous avons utilisé un marqueur microsatellite pour analyser les contributions parentales dans deux croisements contrôlés chez l'huître creuse Crassostrea gigas. 5 mâles et 5 femelles, hétérozygotes à ce locus, et portant chacun des allèles différents (soit 20 allèles ségrégés au total) ont été croisés de deux manières différents: soit en mélangeant toutes les gamètes mâles d'une part, toutes les gamètes femelles d'autre part, et en réalisant un croisement entre ces deux mélanges; soit en fécondant séparément chacune des 5 femelles par chacun de 5 mâles, soit 25 croisement séparés. Pour le deuxième type de... |
Tipo: Text |
Palavras-chave: Contributions parentales; Compétition; Microsatellite; Crassostrea gigas. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00032/14372/11661.pdf |
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Registros recuperados: 81 | |
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